Analysis of real-time PCR Melanocortin 3 (MC3R) gene expression to identify new biomarkers inflammation in tuberculosis

Abstract Background Diagnosis of tuberculosis (TB) in the era technological sophistication requires accuracy and speed to provide as much information possible so that TB treatment can be carried out quickly precisely. New studies have also begun diagnose TB, one which is by examining genes, either looking at polymorphisms, mutations, or expressions. Several previous confirmed association MC3R genes with polymorphisms; a gene participates regulation inflammatory process found macrophages; therefore, we tried analyze expression active group, household contacts, healthy controls for looked differences between three groups correlation seeing group's increased most results considered diagnostic biomarker future. Methods This study included 122 people, 49 patients 46 close relatives patients, 27 controls. used real-time PCR technique groups, all data were analyzed using Bio-Rad CFXTM software version 3.1 one-way ANOVA SPSS 21.0. Results The value group 3.6-fold ( p = 0.143), control 1.09-fold 0.007). Conclusion There relationship based on analysis compared contact